If you are petitioning to get an E-2 Employee, there might only be a 3 month, or 6 month or 1 year limitation. It will depend on the Consulate. Some might even go as far as 5 years.
You might get some visitors from Immigration to make sure the business is real.
Substantive changes must have an amendment filed in order so that you will not be out of status. It must be filed at the California Service Center.